{"id":102,"date":"2017-07-11T13:48:40","date_gmt":"2017-07-11T12:48:40","guid":{"rendered":"http:\/\/www.oceanblogs.org\/m139\/?p=102"},"modified":"2017-07-12T13:34:38","modified_gmt":"2017-07-12T12:34:38","slug":"dry-run-for-the-muc-sample-processing","status":"publish","type":"post","link":"https:\/\/www.oceanblogs.org\/m139\/2017\/07\/11\/dry-run-for-the-muc-sample-processing\/","title":{"rendered":"Dry run for the &#8216;MUC&#8217; sample processing"},"content":{"rendered":"<p>Dear interested reader, colleagues, friends and families,<\/p>\n<p>there are regular presentations given by our scientists on the RV Meteor (Image 1). The first presentation was given by the PhD student Alexandra Sch\u00f6nle from Cologne on her research topic \u201cProtists in the Deep Sea\u201d: How do protists (single-celled eukaryotes, which store DNA in a nucleus) survive the high pressure in the deep sea and which methods are suitable to measure effects of pressure? The next talk will be presented by Drs. Eva Sintes and Chie Amano from Vienna on the activity of prokaryotes (single cells without nucleus or other compartments) in the deep sea.<\/p>\n<p>We are getting closer to the first sampling station, where we will arrive on Wednesday. The sampling of deep-sea sediments will take about one day, requires a detailed protocol and the full attention of our scientists. Therefore, all participants were trained today to handle the valuable multicorer (MUC) cores carefully and quickly (Image 2,3). Each of the MUC cores will be important for different research topics: Which biodiversity can be found in the water and in the sediment, respectively? What are the relative numbers of individuals for certain groups of organisms? Which groups of organisms are really active? It will be interesting, whether the water fraction and the first millimetres of sediment differ concerning our questions. Once we obtain a single MUC core, all organisms will be immediately counted and identified. With this approach, we aim to visualize the complex network of organisms in the deep sea. Our main focus lies on the microbial organisms which are potentially most important for the carbon flux.<\/p>\n<p>Your M139 team<\/p>\n<p>&#8211;<\/p>\n<p>Liebe interessierte Leser, Kollegen, Freunde und liebe Familien,<\/p>\n<p>in regelm\u00e4\u00dfigen Abst\u00e4nden halten unsere Wissenschaftler Vortr\u00e4ge \u00fcber ihre eigene Arbeit auf der FS Meteor (Bild 1). In einem ersten Vortrag informierte uns die K\u00f6lner Doktorandin Alexandra Sch\u00f6nle \u00fcber ihr Thema \u201eProtisten in der Tiefsee\u201c: Wie k\u00f6nnen Protisten (eukaryotische Einzeller, die ihre DNA in einem Zellkern von den anderen Zellbestandteilen durch eine Membran separariert haben) den enormen Druck in der Tiefsee \u00fcberleben und welche geeigneten Methoden gibt es, um die Effekte von Druck auf Protisten zu messen? Die n\u00e4chsten Vortr\u00e4ge werden von Dr. Eva Sintes und Dr. Chie Amano aus Wien \u00fcber die Aktivit\u00e4t von Prokaryoten (Einzeller, die ihre DNA nicht in einem Zellkern konzentriert haben) in der Tiefsee gehalten.<\/p>\n<p>Wir n\u00e4hern uns der ersten Station f\u00fcr Beprobungen, die wir am Mittwoch erreichen werden. Die Beprobung von Tiefsee-Sedimenten wird einen ganzen Tag in Anspruch nehmen und das detaillierte Protokoll wird die volle Aufmerksamkeit unserer Wissenschaftler erfordern. Daher \u00fcbten heute alle Beteiligten, mit den wertvollen Bohrkernen des Multicorer (MUC) sorgf\u00e4ltig und schnell umzugehen (Bild 2,3). Jeder MUC-Bohrkern wird f\u00fcr unterschiedliche Forschungsthemen wichtig sein: Welche Biodiversit\u00e4t wird jeweils im \u00fcberstehenden Wasser und im Sediment zu finden sein? Was sind die relativen Individuenzahlen f\u00fcr die einzelnen Organismengruppen? Welche Organismengruppen sind wirklich aktiv? Es wird interessant sein, ob sich die Proben der Wassers\u00e4ule von denen der ersten Millimeter Sediment hinsichtlich dieser Fragen unterscheiden. Sobald ein einzelner MUC-Bohrkern geborgen wird, werden umgehend alle Organismen gez\u00e4hlt und identifiziert. Wir versuchen mit diesem Ansatz das komplexes Netzwerk an Organismen in der Tiefsee aufzudecken, wobei unser Hauptaugenmerk auf den kleinsten Organismen liegt, die f\u00fcr den Hauptteil des Stoffumsatzes verantwortlich gemacht werden.<\/p>\n<p>Ihr M139-Team<\/p>\n<p>&nbsp;<\/p>\n<div id=\"attachment_103\" style=\"width: 478px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-103\" class=\"wp-image-103 size-medium\" src=\"http:\/\/www.oceanblogs.org\/m139\/wp-content\/uploads\/sites\/52\/2017\/07\/MG_0826-468x312.jpg\" alt=\"\" width=\"468\" height=\"312\" srcset=\"https:\/\/www.oceanblogs.org\/m139\/wp-content\/uploads\/sites\/52\/2017\/07\/MG_0826-468x312.jpg 468w, https:\/\/www.oceanblogs.org\/m139\/wp-content\/uploads\/sites\/52\/2017\/07\/MG_0826-768x512.jpg 768w, https:\/\/www.oceanblogs.org\/m139\/wp-content\/uploads\/sites\/52\/2017\/07\/MG_0826-1024x683.jpg 1024w\" sizes=\"auto, (max-width: 468px) 100vw, 468px\" \/><p id=\"caption-attachment-103\" class=\"wp-caption-text\">Image 1 | Science meeting in the conference room. (Photo: Johannes Werner)<\/p><\/div>\n<p>&nbsp;<\/p>\n<div id=\"attachment_104\" style=\"width: 478px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-104\" class=\"wp-image-104 size-medium\" src=\"http:\/\/www.oceanblogs.org\/m139\/wp-content\/uploads\/sites\/52\/2017\/07\/MG_1142-468x312.jpg\" alt=\"\" width=\"468\" height=\"312\" srcset=\"https:\/\/www.oceanblogs.org\/m139\/wp-content\/uploads\/sites\/52\/2017\/07\/MG_1142-468x312.jpg 468w, https:\/\/www.oceanblogs.org\/m139\/wp-content\/uploads\/sites\/52\/2017\/07\/MG_1142-768x512.jpg 768w, https:\/\/www.oceanblogs.org\/m139\/wp-content\/uploads\/sites\/52\/2017\/07\/MG_1142-1024x683.jpg 1024w\" sizes=\"auto, (max-width: 468px) 100vw, 468px\" \/><p id=\"caption-attachment-104\" class=\"wp-caption-text\">Image 2 | Training of the scientists to handle the multicorer (MUC). The first valuable MUC cores from the deep sea will be obtained on Wednesday. (Photo: Johannes Werner)<\/p><\/div>\n<p>&nbsp;<\/p>\n<div id=\"attachment_105\" style=\"width: 478px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-105\" class=\"wp-image-105 size-medium\" src=\"http:\/\/www.oceanblogs.org\/m139\/wp-content\/uploads\/sites\/52\/2017\/07\/MG_1184-468x702.jpg\" alt=\"\" width=\"468\" height=\"702\" srcset=\"https:\/\/www.oceanblogs.org\/m139\/wp-content\/uploads\/sites\/52\/2017\/07\/MG_1184-468x702.jpg 468w, https:\/\/www.oceanblogs.org\/m139\/wp-content\/uploads\/sites\/52\/2017\/07\/MG_1184-768x1152.jpg 768w, https:\/\/www.oceanblogs.org\/m139\/wp-content\/uploads\/sites\/52\/2017\/07\/MG_1184-683x1024.jpg 683w\" sizes=\"auto, (max-width: 468px) 100vw, 468px\" \/><p id=\"caption-attachment-105\" class=\"wp-caption-text\">Image 3 | Each MUC core has to be processed carefully and quickly. Both the water column and the first millimetres of the sediment will be important for our research. (Photo: Johannes Werner)<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Dear interested reader, colleagues, friends and families, there are regular presentations given by our scientists on the RV Meteor (Image 1). The first presentation was given by the PhD student Alexandra Sch\u00f6nle from Cologne on her research topic \u201cProtists in the Deep Sea\u201d: How do protists (single-celled eukaryotes, which store DNA in a nucleus) survive [&hellip;]<\/p>\n","protected":false},"author":183,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[9],"tags":[],"class_list":["post-102","post","type-post","status-publish","format-standard","hentry","category-deep-sea"],"_links":{"self":[{"href":"https:\/\/www.oceanblogs.org\/m139\/wp-json\/wp\/v2\/posts\/102","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.oceanblogs.org\/m139\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.oceanblogs.org\/m139\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.oceanblogs.org\/m139\/wp-json\/wp\/v2\/users\/183"}],"replies":[{"embeddable":true,"href":"https:\/\/www.oceanblogs.org\/m139\/wp-json\/wp\/v2\/comments?post=102"}],"version-history":[{"count":4,"href":"https:\/\/www.oceanblogs.org\/m139\/wp-json\/wp\/v2\/posts\/102\/revisions"}],"predecessor-version":[{"id":109,"href":"https:\/\/www.oceanblogs.org\/m139\/wp-json\/wp\/v2\/posts\/102\/revisions\/109"}],"wp:attachment":[{"href":"https:\/\/www.oceanblogs.org\/m139\/wp-json\/wp\/v2\/media?parent=102"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.oceanblogs.org\/m139\/wp-json\/wp\/v2\/categories?post=102"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.oceanblogs.org\/m139\/wp-json\/wp\/v2\/tags?post=102"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}